Grapevine-Downy Mildew Rendezvous: Proteome Analysis of the First Hours of an Incompatible Interaction.
Identifieur interne : 000145 ( Main/Exploration ); précédent : 000144; suivant : 000146Grapevine-Downy Mildew Rendezvous: Proteome Analysis of the First Hours of an Incompatible Interaction.
Auteurs : Rita B Santos [Portugal] ; Rui Nascimento [Portugal] ; Ana V Coelho [Portugal] ; Andreia Figueiredo [Portugal]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2020.
Abstract
Grapevine is one of the most relevant crops in the world being used for economically important products such as wine. However, relevant grapevine cultivars are heavily affected by diseases such as the downy mildew disease caused by Plasmopara viticola. Improvements on grapevine resistance are made mainly by breeding techniques where resistance traits are introgressed into cultivars with desired grape characteristics. However, there is still a lack of knowledge on how resistant or tolerant cultivars tackle the P. viticola pathogen. In this study, using a shotgun proteomics LC-MS/MS approach, we unravel the protein modulation of a highly tolerant grapevine cultivar, Vitis vinifera "Regent", in the first hours post inoculation (hpi) with P. viticola. At 6 hpi, proteins related to defense and to response to stimuli are negatively modulated while at 12 hpi there is an accumulation of proteins belonging to both categories. The co-occurrence of indicators of effector-triggered susceptibility (ETS) and effector-triggered immunity (ETI) is detected at both time-points, showing that these defense processes present high plasticity. The results obtained in this study unravel the tolerant grapevine defense strategy towards P. viticola and may provide valuable insights on resistance associated candidates and mechanisms, which may play an important role in the definition of new strategies for breeding approaches.
DOI: 10.3390/plants9111498
PubMed: 33167573
Affiliations:
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type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Grapevine is one of the most relevant crops in the world being used for economically important products such as wine. However, relevant grapevine cultivars are heavily affected by diseases such as the downy mildew disease caused by <i>Plasmopara viticola</i>. Improvements on grapevine resistance are made mainly by breeding techniques where resistance traits are introgressed into cultivars with desired grape characteristics. However, there is still a lack of knowledge on how resistant or tolerant cultivars tackle the <i>P. viticola</i> pathogen. In this study, using a shotgun proteomics LC-MS/MS approach, we unravel the protein modulation of a highly tolerant grapevine cultivar, <i>Vitis vinifera</i> "Regent", in the first hours post inoculation (hpi) with <i>P. viticola</i>. At 6 hpi, proteins related to defense and to response to stimuli are negatively modulated while at 12 hpi there is an accumulation of proteins belonging to both categories. The co-occurrence of indicators of effector-triggered susceptibility (ETS) and effector-triggered immunity (ETI) is detected at both time-points, showing that these defense processes present high plasticity. The results obtained in this study unravel the tolerant grapevine defense strategy towards <i>P. viticola</i> and may provide valuable insights on resistance associated candidates and mechanisms, which may play an important role in the definition of new strategies for breeding approaches.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">33167573</PMID><DateRevised><Year>2020</Year><Month>11</Month><Day>10</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Nov</Month><Day>05</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>Grapevine-Downy Mildew Rendezvous: Proteome Analysis of the First Hours of an Incompatible Interaction.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1498</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants9111498</ELocationID><Abstract><AbstractText>Grapevine is one of the most relevant crops in the world being used for economically important products such as wine. However, relevant grapevine cultivars are heavily affected by diseases such as the downy mildew disease caused by <i>Plasmopara viticola</i>. Improvements on grapevine resistance are made mainly by breeding techniques where resistance traits are introgressed into cultivars with desired grape characteristics. However, there is still a lack of knowledge on how resistant or tolerant cultivars tackle the <i>P. viticola</i> pathogen. In this study, using a shotgun proteomics LC-MS/MS approach, we unravel the protein modulation of a highly tolerant grapevine cultivar, <i>Vitis vinifera</i> "Regent", in the first hours post inoculation (hpi) with <i>P. viticola</i>. At 6 hpi, proteins related to defense and to response to stimuli are negatively modulated while at 12 hpi there is an accumulation of proteins belonging to both categories. The co-occurrence of indicators of effector-triggered susceptibility (ETS) and effector-triggered immunity (ETI) is detected at both time-points, showing that these defense processes present high plasticity. The results obtained in this study unravel the tolerant grapevine defense strategy towards <i>P. viticola</i> and may provide valuable insights on resistance associated candidates and mechanisms, which may play an important role in the definition of new strategies for breeding approaches.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>B Santos</LastName><ForeName>Rita</ForeName><Initials>R</Initials><Identifier Source="ORCID">0000-0001-7809-6813</Identifier><AffiliationInfo><Affiliation>Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nascimento</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>V Coelho</LastName><ForeName>Ana</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-6143-4203</Identifier><AffiliationInfo><Affiliation>Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Figueiredo</LastName><ForeName>Andreia</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0001-8156-7700</Identifier><AffiliationInfo><Affiliation>Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>UIDB/04046/2020</GrantID><Agency>Fundação para a Ciência e a Tecnologia</Agency><Country></Country></Grant><Grant><GrantID>UIDP/04046/2020</GrantID><Agency>Fundação para a Ciência e a Tecnologia</Agency><Country></Country></Grant><Grant><GrantID>PTDC/BIA-BQM/28539/2017</GrantID><Agency>Fundação para a Ciência e a Tecnologia</Agency><Country></Country></Grant><Grant><GrantID>IF/00819/2015</GrantID><Agency>Fundação para a Ciência e a Tecnologia</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>11</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">ETI</Keyword><Keyword MajorTopicYN="N">ETS</Keyword><Keyword MajorTopicYN="N">Plasmopara viticola</Keyword><Keyword MajorTopicYN="N">Vitis vinifera</Keyword><Keyword MajorTopicYN="N">defense-related proteins</Keyword><Keyword MajorTopicYN="N">plant–pathogen interaction</Keyword><Keyword MajorTopicYN="N">proteomics</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>10</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>10</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>11</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>11</Month><Day>10</Day><Hour>1</Hour><Minute>5</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>11</Month><Day>11</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">33167573</ArticleId><ArticleId IdType="pii">plants9111498</ArticleId><ArticleId IdType="doi">10.3390/plants9111498</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Portugal</li></country></list><tree><country name="Portugal"><noRegion><name sortKey="B Santos, Rita" sort="B Santos, Rita" uniqKey="B Santos R" first="Rita" last="B Santos">Rita B Santos</name></noRegion><name sortKey="Figueiredo, Andreia" sort="Figueiredo, Andreia" uniqKey="Figueiredo A" first="Andreia" last="Figueiredo">Andreia Figueiredo</name><name sortKey="Nascimento, Rui" sort="Nascimento, Rui" uniqKey="Nascimento R" first="Rui" last="Nascimento">Rui Nascimento</name><name sortKey="V Coelho, Ana" sort="V Coelho, Ana" uniqKey="V Coelho A" first="Ana" last="V Coelho">Ana V Coelho</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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